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Citation: Li Xiaohong, Zhang Xianming, Ding Xiaobing, Sun Zonghua. Immobolization of Neutral Protease onto Magnetic Polymer Microspheres[J]. Chinese Journal of Applied Chemistry, ;1994, 44(5): 1-4. shu

Immobolization of Neutral Protease onto Magnetic Polymer Microspheres

1.
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041

Received Date: 6 December 1993
Available Online: 23 March 1994
Fund Project:

A neutral protease from Balilius Subtilis has been immobilized onto the magnetic polymer microspheres,which were obtained from styrene,hydroxyethyl methacrylate,divinyl benzene and Fe₃O₄,and had hydroxyl groups in the surface by covalent coupling.Themagnetic immobilized neutral protease had a enzyme activity of 1000U/g,enzyme yield of 20~30mg/g carrier,and activity retention of about 40%.For both free enzyme and immobi-lized enzyme the optimum work temperatures were 50℃ and 50~60℃,the optimum pH values were 7.5 and 6.5,and the kinetic constants were 0.054% and 0.088%,respectively.The thermal stability,pH stability,and the storage stability of the immobilized enzyme are improved obviously.
- magnetic polymer microsphere,
- neutral protease,
- immobilization
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