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Citation: Shi Zuoqing, Zhu Boru, Shi Rongfu, Li Xiaoting, He Binglin, Jia Zhifen, Qiao Jinsheng. Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)[J]. Chinese Journal of Applied Chemistry, ;1996, 13(6): 24-27. shu

Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)

1.
1. Institute of Polymer Chemistry, Nankai University, The State Key Laboratory of Functional Polymer Materials for Ad, Tianjin 300071;
2.
2. Hecheng High Science and Technology Developing Company, Nankai University, Tianjin

Received Date: 30 March 1996
Available Online: 7 August 1996

Anion cellulosic beads were used as support for immobilization of glycosyltransferase GT-1.The immobilized enzyme was used to catalyze the synthesis of α-glycosylstevia sugar.Various influencing factors were discussed.The result showed that when the reaction reached to a certain extent,transglycosylated amount changed very little with increase of amounts of enzyme and starch.The activity of immobilized GT-1 lost very little after repeated uses for 8 times.
- stevia sugar,
- immobilization,
- glycosyltransferase,
- enzyme-catalyzed synthesis
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