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Citation: Zhang Jingshu, Yan Mengmeng, Liu Chunye. Simultaneous Determination of Dissociation Constants of Three Drugs by Internal Standard Capillary Electrophoresis[J]. Chemistry, ;2016, 79(12): 1166-1172. shu

Simultaneous Determination of Dissociation Constants of Three Drugs by Internal Standard Capillary Electrophoresis

1.
College of Pharmacy, Xi'an Medical University, Xi'an 710021

Corresponding author: Liu Chunye,
Received Date: 21 June 2016
Available Online: 1 August 2016
Fund Project:

Simultaneous determination of dissociation constants of three monoprotic weak base drugs, propranolol, atenolol and metoprolol were achieved through internal standard capillary electrophoresis. Three compounds that have similar properties and dissociation constants with the analytes, ephedrine, procaine and diphenhydramine were selected as the internal standards. Precise dissociation constants of the internal standards were deduced using iterative calculation by internal standard capillary electrophoresis. Effective electrophoretic mobilities of the internal standards and the analytes have been determined under the conditions that they are partly or totally protonated. Dissociation constants of the three analytes were further determined simultaneously through single internal standard and multi-internal standards systems, the precision and accuracy of the method were investigated. The relative standard deviation of experimental results were in the range of 0.26%~0.96%, and the obtained values were accurate within 0.1 pK_a uint compared with results of monocomponent determination under the same conditions and the literature values. The technology did not require accurate pH measurement of the buffer solution. Only two electrophoretic runs are needed to complete simultaneous determination of dissociation constants of the three drugs, so the consumed experiment time is shortened significantly. Therefore the proposed method is highly appropriate for high throughput evaluation and screening of drugs in drug development process.
- Capillary electrophoresis,
- Internal standard,
- Dissociation constant
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