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Citation: WANG Feng, LIANG Li-Man, GU Zheng-Biao, CUI Zheng-Gang. Fluorescent Spectrometry of Interaction of Amylase with Polyvinyl Alcohol or Polyethylenamine[J]. Chinese Journal of Applied Chemistry, ;2010, 27(1): 96-101. shu

Fluorescent Spectrometry of Interaction of Amylase with Polyvinyl Alcohol or Polyethylenamine

a.
a. School of Chemical and Meterial Engineering, Jiangnan University, Wuxi 214122;
b.
b. Department of chemisty, Hebei Normal Universiy of Science and Technology, Qinhuangdao;
c.
c. School of Food Science and Technolgy, Jiangnan University, Wuxi

Received Date: 12 January 2009
Available Online: 28 April 2009
Fund Project: 国家高技术研究发展计划("八六三"计划2006AA10Z335) 资助项目 ("八六三"计划2006AA10Z335)

Polyvinyl alcohol(PEG) and polyethylenamine(PEI) have been widely applied in the techno-logical fields related to biochemistry.In this work,the interactions of amylase with PEG and tetraethylene pentamine(TEPA) were investigatied by fluorescence and UV absorption spectra.The results show both the inner fluorescence of amylase and the hydrophobicity of the microenvironment of tyrosine residues are enhanced in the presence of PEG.The quenching mechanism of inner fluorescence of amylase by TEPA is dynamic,but also exhibits a feature of static quenching.The polarity of microenvironment of tryptophan residues of increases amylase due to interaction with TEPA.In the range of this investigation,the binding constant of interaction of PEG with amylase reaches the highest value at 40℃,the value of quenching constant of binding between TEPA and amylase becomes the maximum at 30℃.The interaction of amylse with PEG or TEPA involves hydrophobic and electrostatic interactions.
- PEG,
- TEPA,
- amylase,
- interaction
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