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Citation: YUAN Dao-Qin, FENG Su-Ling, CUI Feng-Ling. Spectroscopic Studies on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, ;2009, 26(8): 918-922. shu

Spectroscopic Studies on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin

1.
College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007

Corresponding author: FENG Su-Ling,
Received Date: 22 July 2008
Available Online: 15 October 2008
Fund Project: 河南省新乡市科技发展项目(07G052) (07G052)

The interaction between diammonium glycyrrhizinate(DG) and bovine serum albumin(BSA) was studied in physicological solution by spectroscopic methods including fluorescence spectrometry and UV-Visible absorption spectrometry.Synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the conformational change of BSA due to the addition of DG.Fluorescence titration reveals that the flurescence intensity of BSA at 348 nm was quenched when DG was added.The dynamic quenching existed between DG and BSA according to the flurescence quenching data at different temperatures and the UV differential spectra.According to Frster theory of non-radiation energy transfer,the nearest distance(r=2.28 nm) and transfer efficiency of energy(E=0.05) between donor(BSA) and acceptor(DG) were determined.The effects of metal ions on the system were studied at room temperature.
- diammonium glycyrrhizinate,
- bovine serum albumin,
- fluorescence spectrum,
- absorption spectrum,
- synchronous fluorescence,
- three-dimensional fluorescence spectrum
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