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Citation: ZHANG Shu-Ran, HU Rong, YANG Qiong, YANG Ji-Dong. Solvent Extraction & Derivative-synchronous Fluorescence Spectrometry on Simultaneous Determination of Methylene Blue and Azure A in Ion-association Complex Form with AuCl₄^-[J]. Chinese Journal of Applied Chemistry, ;2009, 26(7): 811-815. shu

Solvent Extraction & Derivative-synchronous Fluorescence Spectrometry on Simultaneous Determination of Methylene Blue and Azure A in Ion-association Complex Form with AuCl₄^-

ZHANG Shu-Ran ^a ,
HU Rong ^a ,
YANG Qiong ^a ,
YANG Ji-Dong ^a,b,,

1.
a School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715;
2.
b Department of Chemistry, Yangtze Normal University, Chongqing 408100

Corresponding author: YANG Ji-Dong,
Received Date: 21 June 2008
Available Online: 10 September 2008
Fund Project: 重庆市教委科研资助项目(KJ081306) (KJ081306)

A synchronous fluorescence spectrometry combined with first order derivative treatment was deve-loped for the simultaneous determination of methylene blue(MB) and azure A(AA).The method is based upon the formation of ion-association complexes of AuCl₄^- with MB and AA,and the solvent extraction of two complexes by 1,2-dichloroethane in an aciditive medium.When [MB][AuCl₄]₃ and [AA][AuCl₄]₂ complexes were synchronously scanned at Δλ=20 nm and treated with first order derivative techniques in organic phase,the derivative-synchronous fluorescence peaks appeared at 683 nm for [MB][AuCl₄]₃ and at 608 nm for [AA][AuCl₄]₂,respectively.The fluorescence intensities were directly proportional to the concentrations of MB at 683 nm and AA at 608 nm within a certain range.So,a simple,sensitive method has been developed for the simultaneous determination of MB and AA.The detection limit(3σ) and linear range were 7.90×10^-9 g/mL and 3.0×10^-8~4.80×10^-6 g/mL for MB,1.78×10^-8 g/mL and 6.0×10^-8~5.80×10^-6 g/mL for AA,respectively.In this work,the spectral characteristics and compostion of ion-association complexes,the optimum conditions and the influences on the reaction system were investigated.The method can be applied to the simultaneous determination of AA and MB in urine samples and in Azure II with satisfactory results.
- derivative-synchronous fluorescence spectrometry,
- simultaneous determination,
- methylene blue,
- azure A,
- solvent extraction
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Solvent Extraction & Derivative-synchronous Fluorescence Spectrometry on Simultaneous Determination of Methylene Blue and Azure A in Ion-association Complex Form with AuCl4-

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Solvent Extraction & Derivative-synchronous Fluorescence Spectrometry on Simultaneous Determination of Methylene Blue and Azure A in Ion-association Complex Form with AuCl₄^-