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Citation: LI Xiao-Jun, LI Lan, MA Jie, XUE Ying, WU Guo-Hua. Determination of Residual Penicillin in Chicken Muscle by an Electrochemical Immunosensor[J]. Chinese Journal of Applied Chemistry, ;2009, 26(6): 716-720. shu

Determination of Residual Penicillin in Chicken Muscle by an Electrochemical Immunosensor

LI Xiao-Jun ^a ,
LI Lan ^a ,
MA Jie ^a,, ,
XUE Ying ^b ,
WU Guo-Hua ^b

1.
a Department of Chemistry, Capital Normal University, Beijing 100048;
2.
b Beijing Center for Disease Control and Prevention, Beijing

Corresponding author: MA Jie,
Received Date: 5 June 2008
Available Online: 8 October 2008
Fund Project: 北京市自然科学基金(6052002)资助项目 (6052002)

A novel amperometric penicillin immunosensor was prepared by co-immobilizing new methylene blue(NMB) and horseradish peroxidase(HRP)-labeled penicillin polyclonal antibody(Ab^*) on a glassy carbon electrode(GCE) pretreated by electrochemical oxidation and tailored with glutaraldehyde(GA) as the cross-linker through covalent bonding.The quantitative determination of residual penicillin in chicken muscle was investigated through cyclic voltammetry.The experiment results showed that the dependence of current on the concentration of residual penicillin was linear in a range of 5～45 ng/g,and the correlation coefficient was 0.974 7,and the detection limit 1.90 ng/g.The test of the effect of precipitation showed that saturated ammonium sulfate was the best one among the three precipitators.Sample recovery rate was up to 74.5%.At the same time,interference experiment showed that the chicken cross-interference and interference from other components were small.Modified electrode had a good stability and can be used for the determination of penicillin in actual chicken samples.
- immunosensor,
- new methylene blue,
- covalent bonding,
- penicillin
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