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Citation: WAGN Qiu-Yu, QIN Chuan-Guang, ZHANG Qiu-Yu, ZHAO Wen, ZHANG Rui-Jie, LI Yang. Immobilization of Papain by Layer-by-Layer Self-Assembly on Magnetic Polystyrene Microspheres[J]. Chinese Journal of Applied Chemistry, ;2009, 26(5): 557-561. shu

Immobilization of Papain by Layer-by-Layer Self-Assembly on Magnetic Polystyrene Microspheres

WAGN Qiu-Yu ^a,b ,
QIN Chuan-Guang ^a ,
ZHANG Qiu-Yu ^a,b,, ,
ZHAO Wen ^a ,
ZHANG Rui-Jie ^a ,
LI Yang ^a

1.
a Life Science Institute, Northwest Polytechnic University, Xi'an 710072;
2.
b College of Science, Northwest Polytechnic University, Xi'an 710072

Corresponding author: ZHANG Qiu-Yu,
Received Date: 18 May 2008
Available Online: 23 July 2008
Fund Project: 国家自然基金资助项目(20374039) (20374039)

Magnetic polystyrene(PS) microspheres were prepared by layer-by-layer self-assembly technique.The core-shell microspheres were constructed by alternately depositing negative and positive polyelectrolytes onto sulfate-stabilized PS latex particles.And then,papain was immobilized on them.The best circumstance of enzyme immobilization was determined:pH=6.0,1.0 h,30 ℃,mass ratio(papain:microspheres) 37.98%.And the largest ratio of enzyme immobilization could reach 93.0%.The properties of the immobilized enzyme were also studied.The best application temperature and pH were 50 ℃ and 8.0,respectively.When used for 3 times or reserved for 1.5~2.0 h at 80 ℃,the activity of the immobilized papain would fall by nearly half.
- magnetic microsphere,
- layer-by-layer self-assembly,
- enzyme immobilization,
- static adsorption,
- papain
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