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Citation: XIANG Dong-Shan, ZHAI Kun. Determination of Saponins in Methanol Extract from Panax Japonicus by Spectrophotometry[J]. Chinese Journal of Applied Chemistry, ;2009, 26(3): 358-360. shu

Determination of Saponins in Methanol Extract from Panax Japonicus by Spectrophotometry

XIANG Dong-Shan ^a,, ,
ZHAI Kun ^b

1.
a Hubei Key Laboratory of Biological Resources Protection and Utilization, Research Institute of Biological Technology;
2.
b College of Chemistry and Environment Engineering, Hubei Institute for Nationalities, Enshi 445000

Corresponding author: XIANG Dong-Shan,
Received Date: 10 February 2008
Available Online: 11 May 2008
Fund Project: 湖北省自然科学基金(2006ABA036)资助项目 (2006ABA036)生物资源保护与利用湖北省重点实验室开放基金项目(2007020) (2007020)

Saponins of panax japonicus was determined by spectrophotometry at 571 nm wavelength with ginsenoside Rb1 as a reference.The results showed linearity between the quantity and the absorbance,and the linear range of saponins was 100~600 μg.The regression equation was Y=392.47x+104.18(R2=0.998 5).The average recovery rate was 99.54% and the RSD was 0.97%.The method is comparable to HPLC.The method is simple,rapid and accurate.
- panax japonicus,
- saponin,
- spectrometric method,
- determination
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