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Citation: Lv Haijun, Wang Xiaojia, Yu Yifeng, Liu Lei, Hu Zhichao. Study on the Synthetic Conditions of 5-Methyltetrahydrofolate[J]. Chemistry, ;2016, 79(7): 640-644. shu

Study on the Synthetic Conditions of 5-Methyltetrahydrofolate

Lv Haijun ¹ ,
Wang Xiaojia ¹ ,
Yu Yifeng ¹ ,
Liu Lei ^1,2 ,
Hu Zhichao ¹

1.
1. College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018;
2.
2. Hebei Key Laboratory of Molecular Chemistry for Drug, Hebei Research Center of Pharmaceutical and Chemical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018

Received Date: 2 November 2015
Available Online: 26 February 2016
Fund Project:

In this paper, we have established a novel method to synthesize 5-methyltetrahydrofolate (5-MTHF) with high purity and high yield by introducing sodium hyposulfite into the reaction and also substituting citric acid-disodium hydrogen phosphate buffer solution for water. HPLC was used to investigate the influential factors of the reaction, including the addition of sodium salt, reaction temperature, buffer concentration, pH values, etc. The results revealed that, when the folic acid amount was 0.2g, NaBH₄ amount was 0.65g, the reaction temperature was 50℃, the concentration of the buffer was 0.2mol/L, pH value was 6.5 and the addition of formaldehyde was 0.4mL, over 88.9% purity of the target product was obtained with the yield of 70.4%.
- 5-Methyl tetrahydrofolate,
- Sodium hyposulfite,
- High performance liquid chromatography
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