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Citation: Lu Pan, Chai Yahong, Lu Shengjun, Yao Li. Investigating the Binding Force of Thrombin and Its Aptamer Based on Ultra-Low-Field Biological Force Spectroscopy[J]. Chemistry, ;2016, 79(6): 554-557. shu

Investigating the Binding Force of Thrombin and Its Aptamer Based on Ultra-Low-Field Biological Force Spectroscopy

Lu Pan ^1,2 ,
Chai Yahong ² ,
Lu Shengjun ^1,, ,
Yao Li ^2,,

1.
1. College of Materials Science and Metallurgy Engineering, Guizhou University, Guiyang 550025;
2.
2 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190

Corresponding author: Lu Shengjun, Yao Li,
Received Date: 2 January 2016
Available Online: 26 January 2016
Fund Project:

Nucleic acids and proteins are two kinds of most important molecules in cells. Study on their interaction is one of the key issues in molecular biology. Here, we investigated the interaction between thrombin and its corresponding aptamer (T15) based on a new ultra-low-field biological force spectroscopy. The result showed that the binding force of thrombin/aptamer complex is about 80 pN. In addition, the dissociation kinetic information of thrombin/apatamer had been obtained on the molecular level.
- Thrombin,
- Aptamer,
- Interaction force,
- Ultra-low-field biological force spectroscopy
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