Citation: Tang Gang, Yu Xiaodong, Xu Jingjuan, Chen Hongyuan. A Novel Method for Rapid Detection of Thiol Compounds[J]. Chemistry, ;2016, 79(6): 550-553. shu

A Novel Method for Rapid Detection of Thiol Compounds

1.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023

Corresponding author: Yu Xiaodong,
Received Date: 16 December 2015
Available Online: 28 January 2016
Fund Project:

In this work, a novel method for rapid detection of thiol compounds was established using fluorescein isothiocyanate (FITC) as fluorescence probe. The method was based on the quenching of the fluorescence of FITC by thiol compounds. Thiol compounds could be detected in 5 minutes at the optimized conditions including pH and reaction time. The method was used to detect glutathione (GSH) in real samples and showed good selectivity and recovery. The limits of detection (LOD) were 3.08 μmol/L for glutathione (GSH), 2.66 μmol/L for homocysteine (Hcy), 1.28 μmol/L for cysteine (Cys), and 1.92 μmol/L for dithiothreitol (DTT).
- Thiol compounds,
- FITC,
- Fluorescence quench,
- Rapid detection
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