Citation: Zhang Jianqing, Li Ming, Zhang Si, Zhang Feng, Lu Yanan. Study on Preparation and Application of Malachite Green-MIP-96-Wells Detecting Plates[J]. Chemistry, ;2016, 79(4): 366-371. shu

Study on Preparation and Application of Malachite Green-MIP-96-Wells Detecting Plates

1.
Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023

Corresponding author: Zhang Feng,
Received Date: 14 July 2015
Available Online: 13 November 2015
Fund Project:

To detect malachite green (MG) in the aquatic product easily and sensitively, molecularly imprinted polymer (MIP) microspheres were prepared via in-situ polymerization method with MG as template, α-methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and acetonitrile/toluene (V/V=3:1) solution as solvent. Then the MG-MIP-96-well detecting plates detected by the method of chemilumineseent immunoassay (CLIA) were developed by coating the MIPs in the 96-well plates. The standard curve of as-developed method is y=-29197.49x+1945998.45, R²=0.9416, linearity range is 30~66.64 ng/mL, the LOD is 3.355ng/mL, the intra and inter-assay coefficient of variation are 7.38%~18.07% and 12.45%~17.52%, the intra and inter-assay recovery are 103.41%~144.45% and 100.07%~143.20%, and the crossover rates are 27.97% and 30.97% with specifically detecting the MG's analogue, pararosaniline (PA) and crystal violet (CV). The experiment results showed that the as-developed method has great potential in practical application.
- Malachite green (MG),
- Molecularly imprinted polymer (MIP),
- chemilumineseent immunoassay (CLIA)
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沈阳化工大学材料科学与工程学院沈阳 110142