Citation: Liu Xiaoyuan, Zhao Xiaolong, Sang Qian, Du Junlan, Li Zhilin, Wang Kezhi. Interaction of Thiophene-Appended Ruthenium Complexes with Yeast-RNA[J]. Chemistry, ;2016, 79(5): 430-437. shu

Interaction of Thiophene-Appended Ruthenium Complexes with Yeast-RNA

Liu Xiaoyuan ¹ ,
Zhao Xiaolong ^1,, ,
Sang Qian ¹ ,
Du Junlan ¹ ,
Li Zhilin ^1,, ,
Wang Kezhi ²

1.
1. College of Chemistry and Environmental Science, Hebei University, Baoding 071002;
2.
2. College of Chemistry, Beijing Normal University, Beijing 100875

Corresponding author: Zhao Xiaolong, Li Zhilin,
Received Date: 28 September 2015
Available Online: 27 November 2015
Fund Project:

The interaction of thiophene-appended ruthenium complexes [Ru(bpy)₂(Htip)]Cl₂(1) [Ru(Htip)₂(dppz)]Cl₂(2), [Ru(Htip)₃]Cl₂(3) and [Ru₂(bpy)₄(H₂bipt)]Cl₄(4) {bpy=2,2'-bipyridine, Htip=2-(thiophen-2-yl)-1H-imidazo[4,5-f][1,10] phenanthroline, H₂bipt=2,5-bis[1,10] phenanthrolin[4,5-f]-imidazol-2-yl) thiophene, dppz=dipyrido[3,2-a:2',3'-c] phenazine} with yeast-RNA had been investigated by UV-Vis and emission spectroscopy titrations, steady-state emission quenching and salt effect studies, and the RNA and calf thymus DNA (ct-DNA) binding properties of the complexes had also been compared. The results suggested that this kind of thiophene-containing complexes is the strong intercalators of RNA, and in particular, the RNA binding constants of complex 2 and 3 were obtained to be (2.48±0.02)×10⁷L/mol and (4.04±0.04)×10⁷L/mol, which are larger than their reported DNA binding constants and demonstrate the higher RNA binding affinity of the complexes than that of DNA. These complexes could bind to RNA with high strength not only at low salt concentration but also at high salt concentration, with the binding constants as large as (1.07±0.33)×10⁵, (5.62±0.19)×10⁶, (2.11±0.03)×10⁷ and (3.74±0.45)×10⁵L/mol for complex 1, 2, 3 and 4 at the concentration of 100mmol/L NaCl, respectively. Notably, in contrast to the significant increase of emission intensities for DNA binding of the complex 1 and 2, the fluorescence intensity of 1 decreases evidently upon binding to RNA, and there is almost no fluorescence when 2 is free in aqueous solution or bound to RNA, which indicates that complex 1 and 2 display favorable fluorescence recognition property between RNA and DNA.
- Ruthenium complex,
- Thiophene,
- RNA,
- DNA,
- Luminescence
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