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Citation: Zheng Hong, Li Donghui, Chen Qiuying, Xu Jingou. Synthesis of a Near-IR Cyanine and Its Application in Determination of Biomolecules[J]. Chinese Journal of Applied Chemistry, ;1999, 16(3): 17-20. shu

Synthesis of a Near-IR Cyanine and Its Application in Determination of Biomolecules

1.
The Open Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Department of Chemistry, Xiamen University, Xiamen 361005

Received Date: 18 September 1998
Available Online: 8 March 1999
Fund Project: 国家自然科学基金资助项目(29775021) (29775021)

A novel water soluble near-IR cyanine dye(HMC) was synthesized and its structure was characterized by NMR and MS. The absorption and fluorescence spectral characteristics of HMC in aqueous solution were also studied. It was found that in acidic solution of HMC the presence of protein could cause a hypochromic effect at 776 nm, that was used as a probe for the determination of total protein in human serum. The low fluorescence of in situ HMC dimer caused by cationic surfactant CTAB has been tested as a probe for nucleic acid determination.
- near-IR cyanine dye,
- synthesis,
- spectral characteristic,
- protein,
- nucleic acid determination
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