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Wang Haiyan, Mu Shaolin. Direct Separation of Xanthine Oxidase from Milk Using Polyaniline Film[J]. Chinese Journal of Applied Chemistry,
;1999, 16(3): 13-16.
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Based on the isoelectric point property of enzyme and the doping principle of conducting polymers, xanthine oxidase from the fresh buttermilk has been immobilized and separated by polyaniline film and collected in the phosphate buffer. Xanthine oxidase in fresh buttermilk is doped into polyaniline film polymerized on a glassy carbon electrode to form enzyme electrode. This enzyme electrode has a good bioelctrochemical response to xanthine and a normally kinetic behavior in the enzyme-catalyzed reaction process. The optimum pH of the separated xanthine oxidase is 8.4, very close to that of the commercial xanthine oxidase in aqueous solutions.
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