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Citation: CHI Yan-Hua, ZHUANG Jia, YI Xian-Hua, DONG Fa-Qing, LI Ke-An, LI Na, TONG Shen-Yang. Study on the Interaction of O-{2-[α-(2-Hydroxy-5-sulphophenylazo)-benzylidene] hydrazino} benzoic Acid with Pepsin[J]. Chinese Journal of Applied Chemistry, ;2000, 17(1): 26-30. shu

Study on the Interaction of O-{2-[α-(2-Hydroxy-5-sulphophenylazo)-benzylidene] hydrazino} benzoic Acid with Pepsin

1.
1. Department of Material Science and Engineering, Southwest Institute of Technology, Mianyang 621002;
2.
2. Department of Chem istry, Beijing U niversity, Beijing

Received Date: 24 June 1999
Available Online: 8 October 1999
Fund Project: 国家自然科学基金资助项目(49502025) (49502025)

The interaction of title benzoic acid compound with pepsin in pH 2.15 solution has been studied by UV spectrophotometric method.It is considered that the binding of two compounds is mainly due to the electrostatic force. The influences of acidity, sodium chloride concentration, tem perature, reaction time and other proteins on the interaction have been investigated. The apparent molar absorptivity of the binded compound and the maximum binding value were determined to be ε=3.16×10⁵ L/mol·cm and 100, respectively. The Scatchard model is appropriate for the spectral data treatment.
- hy droxy-sulphopheny lazo-benzylidene-hydrazinobenzoic acid,
- pepsin,
- interaction,
- UV spectrometry
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