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Citation: DAI Guo-Liang, LANG Ai-Dong, LI Jin-Ru, . A Method for Separation of Glucose Oxidase[J]. Chinese Journal of Applied Chemistry, ;2000, 17(2): 171-173. shu

A Method for Separation of Glucose Oxidase

1.
Center of Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080

Received Date: 17 September 1999
Available Online: 21 December 1999
Fund Project: 国家自然科学基金资助课题(29573139) (29573139)

A method for separation of glucose oxidase(GOD) has been developed. A chroma tographic column is filled with an anion exchanger of styrene-divinyl-benzene matrix containing quaternary ammonium salt. The buffered phosphate and acetate solutions were used as eluents. Two GOD components(GOD A and GOD B) were separated by this method. Their enzyme activity and Y potential values were 86.9, 27.3, and -4.3 mV, -6.4 mV, respectively. The simplicity and low cost of the method make it valuable for practical applications.
- glucose oxidase,
- separation,
- enzymatic activity,
- nanocomposite hybrid
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