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Citation: LIU Chang-Xian, WEI Xi-Qin. Determination of Acetaldehyde by Enzymatic Method[J]. Chinese Journal of Applied Chemistry, ;2000, 17(4): 422-423. shu

Determination of Acetaldehyde by Enzymatic Method

1.
Department of Chemistry, Central-South Nationality College, Wuhan 430074

Received Date: 24 November 1999
Available Online: 22 February 2000
Fund Project:

An enzymo-spectrophotometric method was proposed based on the catalytic reaction of acetaldehyde and nicotinamide adenine dinucleotide(NADH) by alcohol dehydrogenase(ADH). The change of the absorbance of NADH was determined for calculating the velocity of the enzymatic reaction. Linear calibration graphs were obtained for acetaldehyde in the range of 0.2~10 mg/L, with a detection limit of 0.16 mg/L. The effects of the pH and interferences from metal cations, formaldehyde and n-butylaldehyde on the reaction were examined. The method was simple, rapid and sensitive.
- acetaldehyde,
- enzymatic determination,
- alcohol dehydrogenase
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