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Citation: QI Chao, DING Lan, LIU Zhi, ZHANG Yu-Jing, ZHAO Da-Qing. Haloperoxidase by Imitation Ⅱ.The Enzymological Properties[J]. Chinese Journal of Applied Chemistry, ;2001, 18(7): 560-562. shu

Haloperoxidase by Imitation Ⅱ.The Enzymological Properties

QI Chao ^a,b ,
DING Lan ^a ,
LIU Zhi ^b ,
ZHANG Yu-Jing ^b ,
ZHAO Da-Qing ^a

1.
a Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022;
2.
b Biochemistry Department, Quartermaster University in PLA, Changchun

Received Date: 19 September 2000
Available Online: 3 January 2001
Fund Project: 国家自然科学基金资助项目(29701005) (29701005)

The meso-tetra(T,T,T,T-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques.The antibody could combine with Mn porphyrin and showed a haloperoxidase activity.The affinities of the antibody for antigen and Mn porphyrin were 4.98×10^-5 mol/L and 7.63×10^-5 mol/L, respectively.When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ_cat for H₂O₂ were 5.127 0×10^-3 mol/L and 0.4834 s^-1, respectively.The enzyme exchange number κ_cat/K_m=94.2851 mol/(L·s).
- complete antigen,
- haloperoxidase,
- meso-tetra(T,T,T,T-O-phenylacetylbenzene)porphyrin,
- enzymological property
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