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Citation: HONG Fa-Shui, WEI Zheng-Gui, ZHAO Gui-Wen. Soaking and Synergistic Extracting Reaction of Chlorophyll from Spinach[J]. Chinese Journal of Applied Chemistry, ;2001, 18(7): 532-535. shu

Soaking and Synergistic Extracting Reaction of Chlorophyll from Spinach

1.
a Department of Biological Science, Suzhou University, Suzhou 215151;
2.
b Department of Chemistry, China University of Science and Technology, Hefei

Received Date: 20 August 2000
Available Online: 27 February 2001
Fund Project: 国家自然科学基金(29805003) (29805003)

The extraction efficiencies of chlorophyll from fresh spinach were compared on soaking method and Arnon methods using various organic solvents.The results showed that the extraction efficiencies of chlorophyll with soaking methods using acetone or ethanol were higher by 21%~38% than that with Arnon method.The extraction efficiencies of chlorophyll with soaking methods by acetone or ethanol were higher than that by methanol or ether.Soaking method using a mixture of acetone and ethanol showed higher(by 39%) efficiency than using acetone or ethanol alone.The soaking method using mixture solution of acetone and ethanol shows synergistic character.The studies of synergistic extraction indicated that the mole ratio of ethanol in mixture solution was 0.5 and synergistic coefficiency R was 1.85 at the maximum of distribution ratio, and coordination number was 1 for acetone, ethanol, and mixture of acetone and ethanol coordinated chlorophyll.
- chlorophyll,
- spinach,
- organic solvent,
- extraction
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