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Citation: QIU Jian-Ding, LIANG Ru-Ping, ZOU Xiao-Yong, CAI Pei-Xiang, ZHANG Bao-Sheng. The Self-assembled HBsAb Monolayer Gold Electrode and Its Application to HBsAg Determination[J]. Chinese Journal of Applied Chemistry, ;2001, 18(9): 705-708. shu

The Self-assembled HBsAb Monolayer Gold Electrode and Its Application to HBsAg Determination

1.
a The School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275;
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b China University of Geoscienc Wuhan;
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c Hospital of Zhongshan University, Guangzhou

Received Date: 30 December 2000
Available Online: 26 April 2001
Fund Project: 广东省博士启动基金资助课题(994128) (994128)

A gold electrode modified by hepatitis B surface antibody covalently bound to selfassembled thiourea monolayer has been fabricated to detect the hepatitis B surface antigen.The selfassembled monolayer was formed by spontaneous adsorption of thiourea on gold surface and used as a substrate to be modified by anti-hepatitis B surface antigen through glutaraldehyde amide reactions.The modification procedure was electrochemically monitored by characterization of the electrode surface.The immunoelectrode and saturated calomel electrode were dipped together into the serum samples diluted by PBS buffer solution (pH=7.4) for the potentiometric determination.More than 87.5% of the results of the human serum samples obtained by this method were in agreement with those obtained by enzyme linked immunosorbent assay(ELISA).Calibration graphs were linear for HBsAg in range of 6~320 μg/L with correlation coefficient of 0 998 and detection limit of 4 μg/L.The immunoelectrode prepared has good stability and high sensitivity.
- hepatitis B surface antigen,
- immunoelectrode,
- self-assembled monolayer,
- thiourea
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