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Citation: LI Wei Guo, ZHAO Da-Qing, NI Jia-Zuan. A Calcium/Calmodulin Dependent Phospholipase A_2 Obtained from Chinese Agkistrodon blomhoffii Ussurensis Venom[J]. Chinese Journal of Applied Chemistry, ;2001, 18(9): 682-684. shu

A Calcium/Calmodulin Dependent Phospholipase A_2 Obtained from Chinese Agkistrodon blomhoffii Ussurensis Venom

1.
Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Received Date: 8 January 2001
Available Online: 8 May 2001
Fund Project: 国家自然科学基金重大项目(29890280)资助课题 (29890280)

The effects of calcium ion and rare earth ions on the activity of a phospholipase A₂(PL₂) obtained from Chinese Agkistrodon blomhoffii Ussurensis venom have been investigated.The results indicated that the activity of snake venom PLA₂ is dependent on the concentration of calcium ion.Calmodulin(CAM), a calcium dependent regulator can also stimulate the activity of PLA₂.La³⁺,Eu³⁺,Dy³⁺,Yb³⁺ showed different levels of inhibitory effects on the PLA₂'s activity mostly due to difference in ion radius.The relative activity of PLA₂ inhibited by La³⁺ or Yb³⁺ at the concentration of 13.5μmol/L was 63.5% and 31.8%, respectively.
- phospholipase A₂,
- calmodulin,
- rare earth ions,
- activity
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