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Citation: LEI Fu-Hou, HUANG Zai-Yin, SHI Xiao-Hong. Immobilization of Polyphenol Oxidase by Coordination with Cu(Ⅱ) in Polyvinyl Alcohol Grafted Glutamic Acid Resin[J]. Chinese Journal of Applied Chemistry, ;2001, 18(11): 881-884. shu

Immobilization of Polyphenol Oxidase by Coordination with Cu(Ⅱ) in Polyvinyl Alcohol Grafted Glutamic Acid Resin

1.
Department of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006

Received Date: 26 December 2000
Available Online: 22 March 2001
Fund Project: 广西民族学院重点资助项目(99HXX00004) (99HXX00004)

The synthesis of the immobilization carrier of polyphenol oxidase(PPO): polyvinyl alcohol grafted glutamic acid resin(PGAR) has been studied and the structure of the PGAR has been characterized by IR and DTA techniques. The results showed that the PPO was immobilized onto the carrier resin through coordination with Cu²⁺, forming PGAR-Cu-PPO. The optimum pH for immobilization was 5.59 and 6.64, and the Michaelis-Menten constant was of 1.70×10^-2 mol/L, both are the same as those for free PPO. The immobilized PPO can be used repeatedly and the activity was found reduced by 80% after being reused 5 times.
- polyphenol oxidase,
- poly(vinyl alcohol-g-glutamic acid),
- immobilization,
- coordination polymer
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