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Citation: WU Dao-Cheng, WAN Ming-Xi, ZHANG Sheng, WU Hong, HU Yun-Yu. Preparation and Property of Bone Morphogenetic Protein-2 Polycyanoacrylate Nanoparticle for Injection[J]. Chinese Journal of Applied Chemistry, ;2002, 19(7): 613-616. shu

Preparation and Property of Bone Morphogenetic Protein-2 Polycyanoacrylate Nanoparticle for Injection

WU Dao-Cheng ^a,c,, ,
WAN Ming-Xi ^a ,
ZHANG Sheng ^b ,
WU Hong ^c ,
HU Yun-Yu ^b

1.
a Department of Biomedical Engineering, Xi'an Jiaotong Universi ty, Xi'an 710038;
2.
b Inst itute of Orthopaedics of PLA, Xij ing Hospital, The Fourth Military Medical University, Xi'an;
3.
c Department of Pharmaceutical Chemistry, The Fourth Military Medical University, Xi'an

Corresponding author: WU Dao-Cheng,
Received Date: 5 December 2001
Available Online: 5 April 2002
Fund Project: 国家自然科学基金资助项目(30170271) (30170271)

The bone morphogenetic protein-2(rhBMP-2) was combined with polycyanoacrylate by improved emulsion polymerization method to give nanoparticles for injection use. Their biological activities were tested both in vivo and in vitro. The process of the recombination preparation was optimized by orthogonal factorization method. The size of the nanoparticle was determined by electronic microscopy. The content of rhBMP-2 and its encapsulation rate were determined by UV spectrophotometry. The proliferation of the cell was measured by MTT assay. The biological activity of the rhBMP-2 polycyanoacrylate nanoparticle was examined by alkaline phosphatase(ALP) coloration method. The results showed that the rhBMP-2 polycyanoacrylate nanoparticle was homogenous and stable with the average diameter (107±12) nm and rhBMP encapsulation rate of 78%. The in vivo experiment showed that 80% of rhBMP could be released in 10 days. The ALP level in the marrow stromal cell(MSC) cultural system increased significantly(P<0.01) by adding rhBMP nanoparticle to MSC system.
- bone morphogenetic protein-2,
- polycyanoacrylate,
- encapsalated nanoparticle,
- bone reproduction induction agent,
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