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Citation: GUO Xiao-Ming, WU Xia-Qin, JIANG Li-Ping, ZHANG Zong-Rang. An Improved Sol-Gel Method for Glucose Oxidase Immobilization[J]. Chinese Journal of Applied Chemistry, ;2002, 19(8): 789-791. shu

An Improved Sol-Gel Method for Glucose Oxidase Immobilization

1.
Department of Chemistry, Shanghai Teachers University, Shanghai 200234

Corresponding author: WU Xia-Qin,
Received Date: 5 December 2001
Available Online: 26 March 2002
Fund Project: 上海市教委科技发展基金资助项目(01D04-2) (01D04-2)

An improved sol-gel method was proposed for glucose oxidase immobilization. Gelatin film was introduced onto the surface of the substrate electrode before the formation of the sol-gel membrane. The condition parameters affecting the sol-gel membrane preparation have been investi-gated and optimized. The covered gel membrane with entrapped enzyme was aged in a vessel at 4℃ for 48 h in order to prevent the membrane cracking and peeling off from the surface of substrate electrode. The fabricated glucose sensors exhibited sensitivity of 50 nA/(mmol·L^-1) with linear range in range of 0.05~12 mmol/L, response time 1.5 min and satisfactory lifetime.
- glucose oxidase,
- sol-gel method,
- gelatin,
- biosensor
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