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Citation: WEI Shou-Lian, LI Feng-Ping, MO Jin-Yuan. Quantitative Determination of Berberrabine and Tetrahydroberberine by Combined Capillary Electrophoresis/Conductivity Measurements[J]. Chinese Journal of Applied Chemistry, ;2003, 20(2): 121-124. shu

Quantitative Determination of Berberrabine and Tetrahydroberberine by Combined Capillary Electrophoresis/Conductivity Measurements

1.
a Department of Light Industry & Chemistry, Zhaoqing University, Zhaoqing;
2.
b Department of Chemistry, Zhongshan University, Guangzhou 510275

Corresponding author: MO Jin-Yuan,
Received Date: 26 April 2002
Available Online: 18 November 2002
Fund Project: 国家自然科学基金(29675033) (29675033)广东省自然科学基金(001237) (001237)广西壮族自治区自然科学基金(9912001)资助项目 (9912001)

Berberrabine(BRB) and tetrahydroberberine(THB) were determined simultaneously by combined capillary electrophoresis/conductivity measurements. The effects of pH value and concentration of the running buffer on the determinations were studied. Berberrabine and tetrahydroberberine could be completely separated in a solution of 25 mmol/L Tris with 25 mmol/L citric acid(pH=4.0) within 10 min. An excellent linearity was found between peak area and concentration of analytes in the concentration range of 1.0×10^-5~2.5×10^-3 mol/L for berberrabine and 8.0×10^-6~1.0×10^-3 mol/L for tetrahydroberberine, respectively, r>0.998 for all analytes. The average recovery was 96.8% and 99.2% for berberrabine and tetrahydrober-berine, respectively. Satisfactory results were obtained in analysis of practical berberrabine and tetrahydroberberine samples.
- berberrabine,
- tetrahydro berberine,
- capillary electrophoresis conductivity detection
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