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Citation: XIA Hai-Tao, LIU Yu-Fen, XUE Hong-Yan, CHEN Hong-Wei. Analysis of Soybean Phospholipids Molecular Species by HPLC Using Evaporative Light Scattering Detector[J]. Chinese Journal of Applied Chemistry, ;2003, 20(3): 246-249. shu

Analysis of Soybean Phospholipids Molecular Species by HPLC Using Evaporative Light Scattering Detector

1.
a Institute of Chemistry and Chemical Engineering, Qiqihaer University, Qiqihaer 161006;
2.
b College of Bioengineering, Changchun University of Technology, Changchun

Corresponding author: XIA Hai-Tao,
Received Date: 24 July 2002
Available Online: 29 November 2002

Soybean phospholipids were separated into phospholipids on a semi-preparative silica gel column using HPLC. Phosphatidylcholine(PC), phosphatidylethanolamine(PE) and phosphatidylinositol(PI) fractions were collected. Their molecular species compositions were analysed on a revers phase Prodigy ODS(3) column and a gradient elution program performed with chloroform-methanol-acetonitrile-water as a flow phase. Phosphatidylcholine, phosphatidy lethanolamine and phosphatidylinositol were separated into 11, 9 and 10 molecular species respectively. The molecular species identities were assigned from fatty acid compositions determined by gas chromatography (GC). The method has advantages of simplicity and availability of solvents.
- soybean,
- phospholipid,
- molecular species,
- high performance liquid chromatography,
- evaporative light scattering detector
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