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Citation: SHAO Cheng-Bin, ZHENG Xu-Xu, LI Xin-Ping. Superoxide Dismutase from Ryegrass Leaves (Lolium perenneperennial)[J]. Chinese Journal of Applied Chemistry, ;2003, 20(5): 474-478. shu

Superoxide Dismutase from Ryegrass Leaves (Lolium perenneperennial)

1.
a College of Environmental and Bio-engineering, Chongqing University of Industry and Commerce, Chongqing 400033;
2.
b College of Bio-engineering, Chongqing University, Chongqing

Corresponding author: SHAO Cheng-Bin,
Received Date: 18 September 2002
Available Online: 20 January 2003
Fund Project: 重庆市科委攻关项目(20011812) (20011812)

Superoxide dismutase (SOD) separated and extracted from ryegrass leaves (Lolium perenneperennial) was purified by fraction precipitation by ammonium sulfate and column chromatography.The purified enzyme is found to be a copper/zinc superoxide dismutase as assayed by its sensitivity to KCN and H₂O₂.Electrophoretic analysis indicated that the enzyme contains two homogenous subunits with molecular weight of 15900 with N-terminal being alanine and maximum ultraviolet absorption at 254 nm.The SOD from ryegrass leaves is thermal stable with activity loss of 27% at 65℃ for an hour.Quantitative analysis of amino acids revealed that this protein was composed of 212 amino acids and lack of tryptophan.
- superoxide dismutase,
- ryegrass leaves
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