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Citation: PAN Hong-Cheng, JIANG Zhi-Liang, YUAN Wei-En, TANG Guo-Shun, LUO Yang-He. Fluorescence and Resonance Scattering Spectral Behavior of HSA-Acetone(Ethanol) Systems[J]. Chinese Journal of Applied Chemistry, ;2003, 20(7): 660-663. shu

Fluorescence and Resonance Scattering Spectral Behavior of HSA-Acetone(Ethanol) Systems

1.
Department of Resource Environmental Science, Guangxi Normal University, Guilin 541004

Corresponding author: JIANG Zhi-Liang,
Received Date: 4 December 2002
Available Online: 2 April 2003
Fund Project: 广西自然科学基金(0144003) (0144003)

The fluorescence and resonance scattering spectra of Tris-HCl-acetone (or ethanol)-HSA(human serum albumin) system(pH=7.4) were studied by a double-cell method. The results demonstrate the fluorescence quenching effect of acetone on the HSA system at 325 nm primarily due to the molecular absorption of acetone. The fluorescence intensity of HSA system at 325 nm was observerved enhanced by addition of ethanol. It could be explained in term of the destruction of high structures of HSA by ethanol and the formation of H-bondings from Trp exposed on the HSA surface. The destructed HSA species could be congregated in NaAC-HAC buffer solution of pH=4.8, when the concentration of acetone is >30% or that of ethanol is>40%. The congregation of HSA hurriedly enhances the resonance scattering intensity of the system at 470 nm.
- acetone,
- ethanol,
- HSA,
- fluorescence spectra,
- resonance scattering spectra
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