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Citation: CHEN Shou-Hui, CUI Xiao-Qiang, YANG Fan, NI Yong-Nian, YANG Xiu-Rong. Study of Interaction Between Biotin and Its Monoclonal Antibody by Surface Plasma Resonance(SPR)[J]. Chinese Journal of Applied Chemistry, ;2004, 21(3): 266-270. shu

Study of Interaction Between Biotin and Its Monoclonal Antibody by Surface Plasma Resonance(SPR)

1.
a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022;
2.
b Department of Chemistry, Nanchang University, Nanchang

Corresponding author: YANG Xiu-Rong,
Received Date: 22 June 2003
Available Online: 25 November 2003
Fund Project: 国家自然科学基金资助项目(20075027) (20075027)国家重点基础研究规划项目(2001CB5102) (2001CB5102)

Avidin and biotin are widely used in biology due to the high affinity between them. In some cases anti-biotin antibodies are used in stead of avidin. In this paper, the kinetic characters of anti-biotin antibody and biotinylated bovine serum albumin(BSA) were studied by biomolecular interaction analysis(BIA) technique based on surface plasma resonance(SPR). The association rate constant, dissociation rate constant and affinity constant between anti-biotin antibody and biotinylated BSA were found to be 2.59×10⁴L/(mol·s), 3.77×10^-3s^-1 and 6.86×10⁶ L/mol, respectively.
- anti-biotin,
- kinetics,
- immunoassay,
- surface plasma resonance
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