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Citation: WEI Yong-Feng, MA Dong-Mei, LI Xiao-Hua. Chitosan-immobilized Urease and Analysis of Urea in Waste Water[J]. Chinese Journal of Applied Chemistry, ;2004, 21(4): 357-360. shu

Chitosan-immobilized Urease and Analysis of Urea in Waste Water

1.
Department of Chemistry, Northwest University, Xi'an 710069

Corresponding author: WEI Yong-Feng,
Received Date: 6 June 2003
Available Online: 5 September 2003
Fund Project: 陕西省自然科学基金(01JK073) (01JK073)

The porous chitosan microspheres were prepared. Their activation and immobilization with urease were investigated. It is found that the immobilized urease exihibited storage stability better than the free urease, but with lower activity. The chitosan-immobilized uease was applied as a bioreactor in the determination of urea in waste water by flow injection analysis. The linear range of urea concentration was 1.0×10^-5~5.0×10^-3 g/mL with the mean recovery being (100.5±3.2)%, detection limit was 1.0×10^-5g/mL
- urease,
- chitosan,
- immobilization,
- bioreactor,
- urea determination
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