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Citation: PENG Mi-Jun, ZHOU Chun-Shan, DONG Chao-Qing, ZHONG Shi-An, LIU Bin. Separation and Preparation of Iridoids by Preparative Liquid Chromatography[J]. Chinese Journal of Applied Chemistry, ;2004, 21(5): 455-458. shu

Separation and Preparation of Iridoids by Preparative Liquid Chromatography

1.
a College of Chemistry and Chemical Engineering, Central South University, Changsha 410083;
2.
b Jishou University, Zhangjiajie

Corresponding author: ZHOU Chun-Shan,
Received Date: 27 June 2003
Available Online: 25 September 2003
Fund Project: 国家科技部农业科技成果转化资金计划(02EFN214301161) (02EFN214301161)湖南省科技厅攻关计划项目(00NKY1010) (00NKY1010)湖南省科技厅项目(03NKY2019) (03NKY2019)

Three kinds of iridoid compounds:geniposidic acid(GPA), aucubin(AU) and geniposide(GP) were separated in pure form from the extract of eucommia ulmiodes simultaneously by preparative liquid chromatography. The elution mode, the flow-rate and the composition of the mobile phase including the content of the additive were investigated. A gradient flow rate elution mode was proposed. The optimum condition of the separation of the mixture as follows:mobile phase ethanol-water-formic acid(volume ratio 18:82:0.09), the reverse C₁₈ column was eluted by gradient flow rate, 30 mL/min followed with 55 mL/min. 95.61% of AU, 98.22% of GPA and 98.54% of GP were obtained respectively.
- preparative liquid chromatography,
- iridoid compound,
- separation
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