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Citation: MA Tong-Sen, MA Wu-Sheng, YANG Sheng-Yu. Preparation of Acrylamide Using Resting Cells[J]. Chinese Journal of Applied Chemistry, ;2004, 21(7): 727-730. shu

Preparation of Acrylamide Using Resting Cells

1.
a College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475001;
2.
b College of Life Science, Henan University, Kaifeng 475001

Corresponding author: MA Tong-Sen,
Received Date: 20 August 2003
Available Online: 1 February 2004

The resting cells were used to produce acrylamide from acrylonitrile by enzyme hydration in semi-continuous process.The result indicated that the enzyme Nocardia sp was deactivated at temperature more than 20℃,it was also intensely inhibited when the volume fraction of acrylonitrile was above 4% and product concentration above 30%.The enzyme activity could be kept in considerable level when acrylonitrile and enzyme were added in stream-form and at low temperature.When volume fraction of enzyme was 10% and at 20℃ by this addition mode acrylamide could be obtained in mass concentration of 23% more than that by batch-mode addition process
- resting cell,
- nitrile hydratase,
- acrylamid
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