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Citation: GAO Feng, DU Jun, ZHANG Li, BIAN Gui-Rong, ZHU Chang-Qing, WANG Lun. Reaction of Phenosafranine with Anionic Surfactants and Fluorescence Spectrometric Determination of DNA[J]. Chinese Journal of Applied Chemistry, ;2004, 21(7): 722-726. shu

Reaction of Phenosafranine with Anionic Surfactants and Fluorescence Spectrometric Determination of DNA

1.
College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000

Corresponding author: WANG Lun,
Received Date: 20 August 2003
Available Online: 31 December 2003
Fund Project: 安徽省教育厅基金(2003kj144) (2003kj144)安徽省高校青年教师基金(2003jq104) (2003jq104)

The fluorescence spectra and visible spectra of a cationic fluorescent dye,phenosafranine (PF),in the solution of anionic surfactants has been studied.A non-fluorescent dye dimer with a new characteristic peak at 515 nm was formed when the concentration of anionic surfactant was in the range of 8.0×10^-4~2.0×10 ^-3 mol/L.The studies on the possibility of using the in situ dimmer of dye as a fluorescence probe showed that the fluorescence intensities increased significantly and varied linearly with the concentration of nucleic acid when appropriate amounts of nucleic acid was added,thus a new method for the determination of nucleic acid was realized.Upon optimal conditions,the linear range for ct-DNA,fs-DNA,and yeast-RNA was 0.06~18 mg/L,0.04~20 mg/L and 0.12~20 mg/L,respectively;with detect limit being 21 μg/L,17 μg/L and 36 μg/L,respectively.
- phenosafranine,
- anionic surfactant,
- nucleic acid,
- fluorescence analysis
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