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Citation: DENG Cai, GONG Yue-Fa. Preparation of Dendritic Silica Composite Carriers and Immobilization of Lipase Thereon[J]. Chinese Journal of Applied Chemistry, ;2004, 21(10): 1024-1028. shu

Preparation of Dendritic Silica Composite Carriers and Immobilization of Lipase Thereon

DENG Cai ,
GONG Yue-Fa ^,

1.
Department of Chemistry, Huazhong University of Scienceand Technology, Wuhan 430074

Corresponding author: GONG Yue-Fa,
Received Date: 18 November 2003
Available Online: 10 March 2004

Five dendritic composite silica carriers were prepared and tested in immobilization of porcine pancreas lipase(PPL). The experimental results showed that the content of amino groups on the carrier surface, the chain length and the properties of organic groups have important influence on immobilization efficiency of PPL and the relative activity of immobilized lipase. Among all the carriers, 0.5G' composite carrier showed the best efficiency in immobilizing PPL mainly due to its highest content(1.646 mmol/g) of amino group on the surface. The activity of immobilized lipase is obviously raised with the increase of the organic chain length and the content of amino group (1.5G >0.5G;2.0G >1.0G). The activity of immobilized lipase improved by 35.4% and 13.1%, respectively, when the carriers 0.5G and 1.5G were modified by hydrolysis, indicating that the carboxyl groups on the surface of the carrier favor the immobilization of lipase to the ester groups. The lipase immobilized on 0.5G' composite carrier showed the best activity at 40℃ and in the buffer of pH 7.5. The immobilization conditions were optimized, and the best stirring time was 30 min, and the optimal mass ratio of carrier to lipase about 1 000:75.
- porcine pancreas lipase,
- dendritic composite silica carrier,
- immobilization,
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