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Citation: CHEN Guang-De, ZHANG Yan-Hua. Determination of Proteins in Pigments of Dunhuang Wall Painting by Resonance Light Scattering with Bromophenol Blue as a Probe[J]. Chinese Journal of Applied Chemistry, ;2005, 22(5): 498-501. shu

Determination of Proteins in Pigments of Dunhuang Wall Painting by Resonance Light Scattering with Bromophenol Blue as a Probe

CHEN Guang-De ^, ,
ZHANG Yan-Hua

1.
College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070

Corresponding author: CHEN Guang-De,
Received Date: 26 July 2004
Available Online: 19 September 2004
Fund Project: 国家文物局文物保护基金资助项目(9908) (9908)

Resonance Light Scattering (RLS) probe technique was applied in the quantitative determination of proteins in the binding media of different dyes in Dunhuang Wall Painting via the interaction between Bromophenol Blue (BPB) and the proteins.In the Britton-Robinson buffer solution (pH=4.06),BPB combines with proteins via intermolecular forces,resulting in resonance light scattering.Bovine serum albumin (BSA) was employed as an analogue of the proteins in the wall paintings.It was found that RLS intensity at 345 nm is proportional to the concentration of the proteins in the range of 0.34×10^-6~18.30×10^-6 g/mL.The method is suitable for the determination of proteins in the binding media of the wall paintings,and 9.393 8×10^-6 g/mg),4.868 8×10^-6 g/mg),1.945 4×10^-6 g/mg and 1.092 0×10^-6 g/mg proteins were detected respectively in the red,white,black and green pigments of the wall paintings.The method is simple,rapid and sensitive.The relative standard deviation was 3.1%.The standard addition recovery rate of proteins was between 95.63% and 104.68%.
- Dunhuang wall painting,
- pigment binder,
- resonance light scattering,
- protein,
- bromophenol blue
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