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Citation: FENG Yu-Ying, WU Xiao-Hong, ZHOU Jia-Hong, GU Xiao-Tian, LU Tian-Hong, WANG Xue-Song, ZHANG Bao-Wen. Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods[J]. Chinese Journal of Applied Chemistry, ;2005, 22(8): 895-898. shu

Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods

1.
a Jiangsu Research Center of Bio-medical Functional Materials Engineering, Nanjing Normal University, Nanjing 210097;
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b Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun;
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c Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing

Corresponding author: ZHOU Jia-Hong,
Received Date: 26 October 2004
Available Online: 9 December 2004
Fund Project: 江苏省教育厅自然科学基金(2004191XGQ2B43,04KJD150110) (2004191XGQ2B43,04KJD150110)

The interaction of horse heart hemoglobin with Hypocrellin A (HA) was studied by means of UV-Vis absorption spectroscopy and fluorescence spectroscropy under physiological condition.In the UV-Vis absorption spectrum,the intensity of the absorption peak of amino acid residues in the hemoglobin molecule decreases and the peak shifts bathochromically due to the hydrogen bonding between the amino acid residue in the hemoglobin molecule and HA.The synchronous fluorescence spectra show that the degree of the interaction of HA with different amino acid residues are different.For example,the fluorescence quenching kinetic constants of HA for tryptophane and tyrosine residues were 5.5×10¹² and 1.7×10¹² L/mol,respectively,which clearly demonstrates that the interaction between HA and tryptophane residue is more intense than that between HA and tyrosine residue.
- horse heart hemoglobin,
- Hypocrellin A,
- interaction,
- UV-Vis absorption spectroscopy,
- fluorescence spectroscopy,
- fluorescence quenching kinetic constant
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