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Citation: LI Chun-Ying, ZHAO Chun-Jian, ZU Yuan-Gang. Simultaneous Determination of Solanesol and Coenzyme Q₁₀ in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC[J]. Chinese Journal of Applied Chemistry, ;2006, 23(5): 524-527. shu

Simultaneous Determination of Solanesol and Coenzyme Q₁₀ in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC

1.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Corresponding author: ZU Yuan-Gang,
Received Date: 19 August 2005
Available Online: 8 December 2005
Fund Project: 科技部科研条件升级改造项目(JG-2004-09)资助 (JG-2004-09)

The simultaneous determination of solanesol and coenzyme Q₁₀ in the extract of tobacco(Nicotiana Tabacum L.) leaves by using reversed phase high performance liquid chromatography(RP-HPLC) method was established.The sample preparation process involved homogeneous extraction.The chromatogram was obtained on an HIQ SIL C₁₈V column(4.6 mm×250 mm,5 μm) with V(acetonitrile):V(isopropanol)=8:7 as the mobile phase at a flow rate of 1.5 mL/min and 25℃.Diode array detection(DAD) was used and UV detection wave wavelength was set at 215 nm for solanesol and 275 nm for coenzyme Q₁₀. The volume of injection sample was 20 μL.Under the chromatographic conditions,the recoveries of solanesol and coenzyme Q₁₀ were(100.05±1.25)% and (99.97±1.36)% respectively.The method is simple,rapid,efficient and it is suitable to the determination of solanesol and coenzyme Q₁₀ in the extract of tobacco leaves.
- RP-HPLC,
- DAD,
- tobacco leaves,
- solanesol,
- coenzyme Q₁₀
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Simultaneous Determination of Solanesol and Coenzyme Q₁₀ in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC