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Citation: ZHANG Hong-Mei, WANG Yan-Qing, ZHANG Gen-Cheng. Study of Interaction between Theophylline and Bovine Serum Albumin by Fluorescence Spectroscopy[J]. Chinese Journal of Applied Chemistry, ;2006, 23(8): 866-870. shu

Study of Interaction between Theophylline and Bovine Serum Albumin by Fluorescence Spectroscopy

1.
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers College, Yancheng 224002

Corresponding author: ZHANG Gen-Cheng,
Received Date: 23 September 2005
Available Online: 16 February 2006
Fund Project: 江苏省滩涂生物资源与环境保护重点建设实验室资助项目(JLCBE05032) (JLCBE05032)

The interaction between theophylline and bovine serum albumin(BSA) was investigated via fluorescence spectroscopy.The experimental results show that the fluorescence quenching of BSA by theophylline is due to the formation of theophylline-BSA complex through both static quenching and nonradiative energy transfer. The binding site number n and the apparent binding constant K_A were measured;the binding distance r and the energy transfer efficiency E between theophylline and BSA were obtained according to fluorescence resonance energy transfer;the effect of theophylline on the conformation of BSA was analyzed by means of synchronous fluorescence spectroscopy.
- theophylline,
- bovine serum albumin,
- fluorescence quenching,
- energy transfer
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