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Citation: KANG Tian-Fang, LIU Run, LU Li-Ping, CHENG Shui-Yuan. Biosensors Based on Acetylcholinesterase Immobilized on Regenerated Silk Fibroin[J]. Chinese Journal of Applied Chemistry, ;2006, 23(10): 1099-1103. shu

Biosensors Based on Acetylcholinesterase Immobilized on Regenerated Silk Fibroin

1.
College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100022

Corresponding author: KANG Tian-Fang,
Received Date: 12 November 2005
Available Online: 10 March 2006
Fund Project: 国家重点基础研究发展计划"九七三"项目(2005CB724201) (2005CB724201)国家自然科学基金(20247002) (20247002)北京市教育委员会科技发展基金(Km200310005008)资助项目 (Km200310005008)

An amperometric biosensor for the determination of organophosphorus and carbamate pesticides is described.Acetylcholinesterase(AChE) immobilized on regenerated silk fibroin powder was thoroughly mixed with graphite powder,non-conducting epoxy resin,and electronic mediator 7,7,8,8-tetracyanoquinodimethane(TCNQ) to prepare the biosensor.The preparation conditions of the biosensor were optimized.The proposed method exhibited a good reproducibility and recovery ratio.The inhibition versus the logarithm of concentration was linear to omethoate and carbofuran over the concentration ranges of 5.0×10^-5 g/L to 5.0×10^-3 mg/L and 5.0×10^-5 g/L to 1.0 mg/L with the detection limits of 1.9×10^-5 mg/L and 4.7×10^-5 mg/L,respectively.
- biosensor,
- acetylcholinesterase,
- regenerated silk fibroin
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