Home

Citation: LI Xia-Lan, WEI Guo-Dong, WANG Zhao-Jing, CAI E-Na, CHEN Pei-Qing. Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali[J]. Chinese Journal of Applied Chemistry, ;2006, 23(11): 1304-1306. shu

Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali

1.
Department of Bioengineering, Huaqiao University, Quanzhou 362011

Corresponding author: LI Xia-Lan,
Received Date: 24 December 2005
Available Online: 30 March 2006
Fund Project: 2005年国务院侨办基金(05Q0016) (05Q0016)

After extraction with hot water for several times,the leaf mustard was dried and then a polysaccharide named ALP-2 was isolated from the dried leaf mustard with dilute alkali extraction,alcohol precipitation,and DEAE-Sepharose FF column chromatography.ALP-2 was identified by means of cellulose acetate film electrophoresis and HPLC to be a homogeneous polysaccharide,a non-starch polysaccharide without any protein and nucleic acid.Its average molecular weight was 459 750.The analysis by virtue of HPLC after its complete hydrolysis by acid showed that ALP-2 mainly consisted of rhamnose, arabinose,glucose,galactose,and a unknown monosaccharide.
- leaf mustard,
- polysaccharide,
- isolation,
- purification,
- physicochemical characteristics
1. [1]
  Xuewei Qian , Xingwen Sun , Houjin Li , Zhanxiang Liu , Yuan Zheng , Lin Wu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Shuyong Zhang , Jianrong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Recrystallization Experiments. University Chemistry, 2025, 40(5): 66-75. doi: 10.12461/PKU.DXHX202503126
2. [2]
  Nan Wu , Hang Zhang , Lingling Wei , Quan Gu , Haoquan Zheng , Weiqiang Zhang , Rui Cao . Cyanide: Poison or Treasure?. University Chemistry, 2025, 40(7): 177-188. doi: 10.12461/PKU.DXHX202409072
3. [3]
  Kexin Feng , Jie Zhang , Yujia Sun , Qiong Ai , Longchun Li . 乙酰二茂铁和二茂铁甲酰丙酮的合成、纯化及表征. University Chemistry, 2025, 40(8): 307-314. doi: 10.12461/PKU.DXHX202409045
4. [4]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
5. [5]
  Yang Chen , Peng Chen , Yuyang Song , Yuxue Jin , Song Wu . Application of Chemical Transformation Driven Impurity Separation in Experiments Teaching: A Novel Method for Purification of α-Fluorinated Mandelic Acid. University Chemistry, 2024, 39(6): 253-263. doi: 10.3866/PKU.DXHX202310077
6. [6]
  Dong-Bing Cheng , Junxin Duan , Haiyu Gao . Experimental Teaching Design on Chitosan Extraction and Preparation of Antibacterial Gel. University Chemistry, 2024, 39(2): 330-339. doi: 10.3866/PKU.DXHX202308053
7. [7]
  Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
8. [8]
  Longping Li , Jiali Li , Tiange Qu , Jiaqing Cai , Chuyu Zhang , Wenji Guo , Qiulian Li , Fan Luo . “可视化”助力从茶叶中提取咖啡因实验的关键步——升华. University Chemistry, 2025, 40(8): 272-276. doi: 10.12461/PKU.DXHX202409137
9. [9]
  Tao Yang , Kaijiao Duan , Siyu Li , Jing Wei , Qingdi Yang , Qian Wang . A Comprehensive and Innovative Chemical Experimental Teaching: Extraction and Identification of Tea Polyphenols from Pu'er Tea and the Application in Hand Cream Making. University Chemistry, 2024, 39(8): 270-275. doi: 10.3866/PKU.DXHX202312040
10. [10]
  Shu'e Song , Xiaokui Wang , Yongmei Liu , Wanchun Zhu , Hong Yuan , Fuping Tian , Yunshan Bai , Yunchao Li , Li Wang , Zhongyun Wu , Yuan Chun , Jianrong Zhang , Shuyong Zhang . Suggestions on Operating Specifications of Physical Chemistry Experiment: Measurement of Viscosity, Density and Optical Properties. University Chemistry, 2025, 40(5): 148-156. doi: 10.12461/PKU.DXHX202503026
11. [11]
  Fuping Tian , Yunshan Bai , Wanchun Zhu , Yufeng Li , Yongmei Liu , Shu'e Song , Hong Yuan , Zhongyun Wu , Li Wang , Xiaokui Wang , Yuan Chun , Jianrong Zhang , Shuyong Zhang . Suggestions on Operating Specifications of Physical Chemistry Experiment: Measurement of Thermal Properties. University Chemistry, 2025, 40(5): 157-164. doi: 10.12461/PKU.DXHX202503054
12. [12]
  Yunchao Li , Hong Yuan , Yuan Chun , Xiaokui Wang , Fuping Tian , Yunshan Bai , Yongmei Liu , Wanchun Zhu , Shu'e Song , Zhongyun Wu , Li Wang , Yufeng Li , Jianrong Zhang , Shuyong Zhang . Suggestions on Operating Specifications of Physical Chemistry Experiment: Measurement of Electrical Properties. University Chemistry, 2025, 40(5): 165-177. doi: 10.12461/PKU.DXHX202503055
13. [13]
  Yuan Chun , Yongmei Liu , Fuping Tian , Hong Yuan , Shu'e Song , Wanchun Zhu , Yunchao Li , Zhongyun Wu , Xiaokui Wang , Yunshan Bai , Li Wang , Jianrong Zhang , Shuyong Zhang . Suggestions on Operating Specifications of Physical Chemistry Experiment: Measurement of Colloidal and Surface Chemical Properties, Molecular Structure and Properties. University Chemistry, 2025, 40(5): 178-188. doi: 10.12461/PKU.DXHX202503053
14. [14]
  Wanqun Hu , Pingping Zhu , Yuan Zheng , Wanqun Zhang , Wei Shao , Hong Wu , Qiang Zhou , Kaiping Yang , Xiang Sheng . Design and Practice of Ideological and Political Case Study in Instrumental Analysis Experiment Course: the Extraction and Structural Identification of Artemisinin. University Chemistry, 2024, 39(2): 203-207. doi: 10.3866/PKU.DXHX202310062
15. [15]
  Shui Hu , Houjin Li , Zhenming Zang , Lianyun Li , Rong Lai . Integration of Science and Education Promotes the Construction of Undergraduate-to-Master’s Integration Experimental Courses: A Case Study on the Extraction, Separation and Identification of Artemisinin from Artemisia annua. University Chemistry, 2024, 39(4): 314-321. doi: 10.3866/PKU.DXHX202310063
16. [16]
  Tao Cao , Fang Fang , Nianguang Li , Yinan Zhang , Qichen Zhan . Green Synthesis of p-Hydroxybenzonitrile Catalyzed by Spinach Extracts under Red-Light Irradiation: Research and Exploration of Innovative Experiments for Pharmacy Undergraduates. University Chemistry, 2024, 39(5): 63-69. doi: 10.3866/PKU.DXHX202309098
17. [17]
  Huicheng Yang , Qianhong Sun . Practice of Ideological and Political Education in Organic Chemistry Laboratory Courses under the OBE Framework: A Case Study of Berberine Extraction. University Chemistry, 2025, 40(5): 276-282. doi: 10.12461/PKU.DXHX202410091
18. [18]
  Zhanming Zhang , Can Zhu , Juan Wang , Yanghui Lin , Mo Sun . Ideological and Political Cases in the Course of Organic Chemistry Experiment: Taking Caffeine Extraction from Tea Leaves Experiment as an Example. University Chemistry, 2025, 40(7): 34-41. doi: 10.12461/PKU.DXHX202409030
19. [19]
  Meng Lin , Hanrui Chen , Congcong Xu . Preparation and Study of Photo-Enhanced Electrocatalytic Oxygen Evolution Performance of ZIF-67/Copper(I) Oxide Composite: A Recommended Comprehensive Physical Chemistry Experiment. University Chemistry, 2024, 39(4): 163-168. doi: 10.3866/PKU.DXHX202308117
20. [20]
  Bo YANG , Gongxuan LÜ , Jiantai MA . Corrosion inhibition of nickel-cobalt-phosphide in water by coating TiO₂ layer. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 365-384. doi: 10.11862/CJIC.20240063

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(394)
HTML views(25)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return