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Citation: WANG Chuan-Jin, WEI Yun-Yang, ZHU Guang-Jun, DU Yang-Yan. Preparation of High Purity Epigallocatechin Gallate by Polyamide Packed Chromatographic Column Separation[J]. Chinese Journal of Applied Chemistry, ;2007, 24(4): 443-447. shu

Preparation of High Purity Epigallocatechin Gallate by Polyamide Packed Chromatographic Column Separation

1.
School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094

Corresponding author: WEI Yun-Yang,
Received Date: 10 May 2006
Available Online: 8 September 2006

Polyphenols were obtained from green tea by soaking it with ethanol,followed by concentration,decolorization and extraction with ethyl acetate.High purity(-)-EGCG was then obtained by polyamide packed chromatographic column separation.Orthogonal design of experiment was used to determine the optimal amount of alcohol,extraction times and particle diameter of green tea to maximize the extraction yield of(-)-EGCG.The optimal conditions were determined as follows.Twelve mL of 95% alcohol were used to extract 1 g of green tea with particle size less than 0.960 mm for 1 hour and the extraction was repeated 3 times.The decolorization of the ethanol extract of green tea with active carbon was studied.Optimal results of decolorization were obtained by using 0.36 g of activated carbon to decolor 1 g of green tea for 30 min and the decolorization was repeated 2 times.The polyamide chromatography separation conditions of crude polyphenol were investigated.The influence of particle size and amount of polyamide used on extraction yield of(-)-EGCG were studied.It was found that best separation results were obtained if 50 g of polyamide with particle size of 0.170~0.210 mm were used for 1 g extraction of green tea.0.04 g of(-)-EGCG with over 99.0% purity was obtained from 1 g of green tea through optimized process of ethanol extraction,discolo-rization and separation.The extraction yield of(-)-EGCG was 50%.The structure of the final product separated was identified by LC-MS,elemental analysis,UV,IR,HPLC and NMR.It was experimentally demonstrated that the proposed process is a safe,mild,low cost and waste free procedure.
- (-)-EGCG,
- polyamide,
- HPLC,
- extraction process,
- orthogonal design of experiment,
- activated carbon
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