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Citation: WANG Xin, JIA Li-Hua, GUO Xiang-Feng, LU Xiao-Xia. Interaction Between Alpha-Lipoic Acid and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, ;2007, 24(7): 761-764. shu

Interaction Between Alpha-Lipoic Acid and Bovine Serum Albumin

1.
Chemical and Chemistry Engineering College of Qiqihar University, Qiqihar 161006

Corresponding author: JIA Li-Hua,
Received Date: 29 August 2006
Available Online: 29 October 2006
Fund Project: 国家自然科学基金(20572056) (20572056)黑龙江省自然科学基金(E200530) (E200530)

The interaction between alpha-lipoic acid (ALA) and bovine serum albumin (BSA) was studied via fluorescence and ultraviolet-visible absorption spectra in buffer at different temperatures.The results indicate that static quenching was involved in added ALA in BSA solution and the quenching constant (K_SV) was 4.65×10³ L/mol (26℃) and 4.46×10³ L/mol (37℃) respectively.The energy transfer efficiency E is 5% and the distance r is 2.90 nm between drug and serum albumin according to Förster's non-radiation energy transfer.The binding constants is 4.31×10³ L/mol (26℃) and 4.27×10³ L/mol (37℃) respectively and they react at a molar ratio of 1:1.The interaction between alpha-lipoic acid and bovine serum albumin is driven mainly by eletro-static force according to thermodynamic parameters.
- alpha lipoic acid,
- bovine serum albumin,
- fluorescence quenching
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