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Citation: LIANG Yan-Qiu, SUN Peng, LIU Ting-Ting, DENG Bin, ZANG Shu-Liang. Fluorescence Spectra of Binding Reaction Between Cetyltrimethylammonium Bromide and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, ;2007, 24(8): 965-967. shu

Fluorescence Spectra of Binding Reaction Between Cetyltrimethylammonium Bromide and Bovine Serum Albumin

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a Department of Chemistry, EastChina Normal University, Shanghai 200062;
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b Department of Environmental Engineering, Shenyang Institute of Chemical Technology, Shenyang;
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c Department of Chemistry, Liaoning University, Shenyang 110036

Corresponding author: ZANG Shu-Liang,
Received Date: 30 August 2006
Available Online: 24 November 2006
Fund Project: 国家自然科学基金资助课题(20271024) (20271024)

The interaction of cetyltrimethylammonium bromide (CTAB) with bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and synchronous fluorescence under simulative physiological conditions.In the mechanism study,it shows that the fluorescence quenching of BSA by CTAB is static quenching together with the formation of a complex of BSA and CTAB.The binding constant K_A(305 K:7.09×10⁴ L/mol;310 K:4.97×10⁴ L/mol) and thermodynamic parameters (ΔH=-55.85 kJ/mol;ΔS=-90.26 J/(mol·K)/-90.55 J/(mol·K);ΔG=-28.32(kJ/mol)/-27.87(kJ/mol) at different temperatures were calculated according to Lineweaver-Burk equation.The thermodynamic paramters show hydrogen bond and Van der Waals interactions play important roles to stabilize the complex.The result of synchronous fluorescence indicates that the polarity around the tryptophan residues has decreased and the hydrophobicity has increased.
- cetyltrimethylammonium bromide,
- bovine serum albumin,
- binding reaction,
- thermodynamic parameter,
- fluorescence spectroscopy
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