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Citation: FU Yu-Jie, LIU Wei, HOU Chun-Lian, LIU Xiao-Na, LI Shuang-Ming, ZU Yuan-Gang. Simultaneous Determination of Three Flavonoids in Eupatorium Odoratum Linn by RP-HPLC/PAD[J]. Chinese Journal of Applied Chemistry, ;2007, 24(12): 1452-1454. shu

Simultaneous Determination of Three Flavonoids in Eupatorium Odoratum Linn by RP-HPLC/PAD

1.
MOE Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040

Corresponding author: ZU Yuan-Gang,
Received Date: 10 January 2007
Available Online: 17 April 2007
Fund Project: 教育部新世纪优秀人才支持计划(NCET-04-0316) (NCET-04-0316)黑龙江省自然科学基金项目(ZJN04-03)资助 (ZJN04-03)

The contents of luteolin,quercetin and kaempferol in Eupatorium Odoratum Linn were determined with RP-HPLC/PAD method.The chromatographic conditions were chromatographic column HiQ sil C18W,detection wavelength 254 nm for quercetin, 360 nm for luteolin and kaempferol,temperature 30℃,mobile phase CH₃OH-H₂O-H₃PO₄(50:49.8:0.2,volume ratio), 1 mL/min flow rate,10 μL injection volume.Analysis sample was prepared by ultrasonic extraction from Eupatorium Odoratum Linn with the following conditions:85% ethanol solution as solvent,ratio of liquid to solid 10:1(mL/g),extraction time 1 h.The results showed good linearity(R²>0.999 0) for the three flavonoids over a range of 0.01×10^-3~0.10×10^-3 g/mL,and the average recovery yields were 99.601 7%,99.032 6%,and 99.450 8%,respectively.The relative standard deviation(RSD) was less than 2%.The method is simple and efficient for the determination of luteolin,quercetin and kaempferol from Eupatorium odortum Linn with high accuracy.
- Eupatorium odortum Linn,
- flavonoid,
- high performance liquid chromatography(HPLC),
- ultrasonic extraction
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