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Citation: ZHONG Ping. Synthesis of CdSe/CdS Nanocrystals as Fluorescent Probes for Lysozyme[J]. Chinese Journal of Applied Chemistry, ;2007, 24(12): 1428-1432. shu

Synthesis of CdSe/CdS Nanocrystals as Fluorescent Probes for Lysozyme

ZHONG Ping ^,

1.
Department of Biology and Chemistry, Zhanjiang Institute College, Zhanjiang 524037

Corresponding author: ZHONG Ping,
Received Date: 6 January 2007
Available Online: 28 March 2007

CdSe/CdS nanocrystals(NCs) was prepared and modified with 11-mercaptoundecanoic acid [HS-(CH₂)₁₀-COOH] in aqueous solution.The functionalized NCs were characterized via transmission electron microscopy(TEM),ultraviolet-visible(UV-Vis) spectroscopy,and photoluminescence(PL) spectroscopy.The results demonstrate that CdSe/CdS NCs are dispersed homogeneously in aqueous solution and well protected from the environmental oxygen.They can be used as a new fluorescence probe for lysozyme,which is simple,rapid and specific.Under the optimum conditions,linear relationship is found between the relative fluorescence intensity and the lysozyme concentration in a range of 0~44×10^-6 g/mL for CdSe/CdS NCs,the linear equation is ΔF=73+41.9c(10⁶c(lys)/(g·mL-1).The detection limit of CdSe/CdS NCs is 2.33×10^-7 g/mL.The proposed method has been applied to the determination of lysozyme in egg white.
- mercaptoundecanoic acid,
- nanocrystals,
- quantum dots,
- CdSe/CdS,
- lysozyme
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沈阳化工大学材料科学与工程学院沈阳 110142

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Synthesis of CdSe/CdS Nanocrystals as Fluorescent Probes for Lysozyme

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com