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Citation: LI Lei, LIU Xue-Feng, FANG Yun, ZOU Zhu-Yan. Interference of Coexisting Substances in Esculetin-Bovine Serum Albumin Binding Process[J]. Chinese Journal of Applied Chemistry, ;2008, 25(1): 106-110. shu

Interference of Coexisting Substances in Esculetin-Bovine Serum Albumin Binding Process

1.
School of Chemistry & Material Engineering, Jiangnan University, Wuxi 214122

Corresponding author: LIU Xue-Feng,
Received Date: 11 February 2007
Available Online: 21 May 2007

The interference,caused by 6 kinds of coexisting substances,in esculetin-bovine serum albumin(BSA) binding process was investigated for the first time by means of CD,UV-Vis and fluorescence spectroscopy.The parameters such as K_P,K_A,n and r were obtained.The detailed interferential quomodo of the coexisting substances were concluded.With I-,r decreased in the range of 0.3%~2.7%,and K_P and K_A increased in the range of 5.9%~8.1%,and 11.5%~20.6%,respectively;moreover,n increased from 1.58±0.04 to 1.74±0.25,and ion-bridge was the possible interferential quomodo of I-.Sodium Benzoate decreasd r in the range of 2.4%~4.5%,increased K_P in the range of 3.9%~6.9%,and decreased K_A in the range of 1.9%~3.4%,while n decreased from 1.58±0.04 to 0.92±0.16,which suggested that appositional replacement was sodium benzoate's interferential quomodo.SDS increased r in the range of 1.2%~2.8%,decreased K_P and K_A in the range of 11.4%~12.2% and 27.5%~27.8%,respectively,and decreased n from 1.58±0.04 to 1.06±0.12.TW80 decreased r and K_A in the range of 1.2%~2.4% and 10.0%~20.1%,respectively,and decreased K_P in the range of 4.7%~4.9%,and decreased n from 1.58±0.04 to 1.00±0.27.SDS and TW80 probably interfered by destroying the binding site(s).As for TW80,appositional replacement was another probable interferential quomodo.In addition CD results showed that BSA molecular conformational transition caused by the coexisting substances-BSA binding was a common interferential quomodo of the coexisting substances.Furthermore,it was the main interferential quomodo of glucose and VC,too.
- coexisting substance,
- interferential quomodo,
- active component of Traditional Chinese Medicine,
- bovine serum albumin
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