Home

Citation: LI Lei, LIU Xue-Feng, FANG Yun, ZOU Zhu-Yan. Interference of Coexisting Substances in Esculetin-Bovine Serum Albumin Binding Process[J]. Chinese Journal of Applied Chemistry, ;2008, 25(1): 106-110. shu

Interference of Coexisting Substances in Esculetin-Bovine Serum Albumin Binding Process

1.
School of Chemistry & Material Engineering, Jiangnan University, Wuxi 214122

Corresponding author: LIU Xue-Feng,
Received Date: 11 February 2007
Available Online: 21 May 2007

The interference,caused by 6 kinds of coexisting substances,in esculetin-bovine serum albumin(BSA) binding process was investigated for the first time by means of CD,UV-Vis and fluorescence spectroscopy.The parameters such as K_P,K_A,n and r were obtained.The detailed interferential quomodo of the coexisting substances were concluded.With I-,r decreased in the range of 0.3%~2.7%,and K_P and K_A increased in the range of 5.9%~8.1%,and 11.5%~20.6%,respectively;moreover,n increased from 1.58±0.04 to 1.74±0.25,and ion-bridge was the possible interferential quomodo of I-.Sodium Benzoate decreasd r in the range of 2.4%~4.5%,increased K_P in the range of 3.9%~6.9%,and decreased K_A in the range of 1.9%~3.4%,while n decreased from 1.58±0.04 to 0.92±0.16,which suggested that appositional replacement was sodium benzoate's interferential quomodo.SDS increased r in the range of 1.2%~2.8%,decreased K_P and K_A in the range of 11.4%~12.2% and 27.5%~27.8%,respectively,and decreased n from 1.58±0.04 to 1.06±0.12.TW80 decreased r and K_A in the range of 1.2%~2.4% and 10.0%~20.1%,respectively,and decreased K_P in the range of 4.7%~4.9%,and decreased n from 1.58±0.04 to 1.00±0.27.SDS and TW80 probably interfered by destroying the binding site(s).As for TW80,appositional replacement was another probable interferential quomodo.In addition CD results showed that BSA molecular conformational transition caused by the coexisting substances-BSA binding was a common interferential quomodo of the coexisting substances.Furthermore,it was the main interferential quomodo of glucose and VC,too.
- coexisting substance,
- interferential quomodo,
- active component of Traditional Chinese Medicine,
- bovine serum albumin
1. [1]
  Qianlang Wang , Jijun Sun , Qian Chen , Quanqin Zhao , Baojuan Xi . The Appeal of Organophosphorus Compounds: Clearing Their Name. University Chemistry, 2025, 40(4): 299-306. doi: 10.12461/PKU.DXHX202405205
2. [2]
  Tianyun Chen , Ruilin Xiao , Xinsheng Gu , Yunyi Shao , Qiujun Lu . Synthesis, Crystal Structure, and Mechanoluminescence Properties of Lanthanide-Based Organometallic Complexes. University Chemistry, 2024, 39(5): 363-370. doi: 10.3866/PKU.DXHX202312017
3. [3]
  Jingjing QING , Fan HE , Zhihui LIU , Shuaipeng HOU , Ya LIU , Yifan JIANG , Mengting TAN , Lifang HE , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and anticancer activity of two complexes of dimethylglyoxime organotin. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1301-1308. doi: 10.11862/CJIC.20240003
4. [4]
  Liang TANG , Jingfei NI , Kang XIAO , Xiangmei LIU . Synthesis and X-ray imaging application of lanthanide-organic complex-based scintillators. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1892-1902. doi: 10.11862/CJIC.20240139
5. [5]
  Jiaming Xu , Yu Xiang , Weisheng Lin , Zhiwei Miao . Research Progress in the Synthesis of Cyclic Organic Compounds Using Bimetallic Relay Catalytic Strategies. University Chemistry, 2024, 39(3): 239-257. doi: 10.3866/PKU.DXHX202309093
6. [6]
  Aidang Lu , Yunting Liu , Yanjun Jiang . Comprehensive Organic Chemistry Experiment: Synthesis and Characterization of Triazolopyrimidine Compounds. University Chemistry, 2024, 39(8): 241-246. doi: 10.3866/PKU.DXHX202401029
7. [7]
  Bao Jia , Yunzhe Ke , Shiyue Sun , Dongxue Yu , Ying Liu , Shuaishuai Ding . Innovative Experimental Teaching for the Preparation and Modification of Conductive Organic Polymer Thin Films in Undergraduate Courses. University Chemistry, 2024, 39(10): 271-282. doi: 10.12461/PKU.DXHX202404121
8. [8]
  Xilin Zhao , Xingyu Tu , Zongxuan Li , Rui Dong , Bo Jiang , Zhiwei Miao . Research Progress in Enantioselective Synthesis of Axial Chiral Compounds. University Chemistry, 2024, 39(11): 158-173. doi: 10.12461/PKU.DXHX202403106
9. [9]
  .
  CCS Chemistry 综述推荐│绿色氧化新思路：光/电催化助力有机物高效升级
  . CCS Chemistry, 2025, 7(10.31635/ccschem.024.202405369): -.
10. [10]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
11. [11]
  Feng Han , Fuxian Wan , Ying Li , Congcong Zhang , Yuanhong Zhang , Chengxia Miao . Comprehensive Organic Chemistry Experiment: Phosphotungstic Acid-Catalyzed Direct Conversion of Triphenylmethanol for the Synthesis of Oxime Ethers. University Chemistry, 2025, 40(3): 342-348. doi: 10.12461/PKU.DXHX202405181
12. [12]
  Xinxin YU , Yongxing LIU , Xiaohong YI , Miao CHANG , Fei WANG , Peng WANG , Chongchen WANG . Photocatalytic peroxydisulfate activation for degrading organic pollutants over the zero-valent iron recovered from subway tunnels. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 864-876. doi: 10.11862/CJIC.20240438
13. [13]
  Weiliang Wang , Zijing Yu , Jingyuan Li , Hong Shang . The Debate between Traditional Chinese Medicine and Western Medicine. University Chemistry, 2024, 39(9): 109-114. doi: 10.12461/PKU.DXHX202402001
14. [14]
  Tiancheng Yang , Yang Yang , Chunhua Qu , Rui Chu , Yue Xia . Wandering through the Kingdom of Chinese Mineral Medicines. University Chemistry, 2024, 39(9): 94-101. doi: 10.12461/PKU.DXHX202403015
15. [15]
  Cheng Zheng , Shiying Zheng , Yanping Zhang , Shoutian Zheng , Qiaohua Wei . Synthesis, Copper Content Analysis, and Luminescent Performance Study of Binuclear Copper (I) Complexes with Isomeric Luminescence Shift: A Comprehensive Chemical Experiment Recommendation. University Chemistry, 2024, 39(7): 322-329. doi: 10.3866/PKU.DXHX202310131
16. [16]
  Yihao Zhao , Jitian Rao , Jie Han . Synthesis and Photochromic Properties of 3,3-Diphenyl-3H-Naphthopyran: Design and Teaching Practice of a Comprehensive Organic Experiment. University Chemistry, 2024, 39(10): 149-155. doi: 10.3866/PKU.DXHX202402050
17. [17]
  .
  南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积
  . CCS Chemistry, 2025, 7(0): -.
18. [18]
  Yuan Zheng , Quan Lan , Zhenggen Zha , Lingling Li , Jun Jiang , Pingping Zhu . Teaching Reform of Organic Synthesis Experiments by Introducing Reverse Thinking and Design Concepts: Taking the Synthesis of Cinnamic Acid Based on Retrosynthetic Analysis as an Example. University Chemistry, 2024, 39(6): 207-213. doi: 10.3866/PKU.DXHX202310065
19. [19]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
20. [20]
  Zongfei YANG , Xiaosen ZHAO , Jing LI , Wenchang ZHUANG . Research advances in heteropolyoxoniobates. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 465-480. doi: 10.11862/CJIC.20230306

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(365)
HTML views(42)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return

Interference of Coexisting Substances in Esculetin-Bovine Serum Albumin Binding Process

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

Metrics

通讯作者: 陈斌, bchen63@163.com