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Citation: WANG Yan, YAO Li-Li, ZHOU Lin, DAI Shan. Preparation of Pyruvate from DL-Lactate by Immobilized Lactate Oxidase on Calcium Alginate[J]. Chinese Journal of Applied Chemistry, ;2008, 25(4): 489-493. shu

Preparation of Pyruvate from DL-Lactate by Immobilized Lactate Oxidase on Calcium Alginate

1.
School of Chemistry and Chemical Engineering, Central South University, Changsha 410083

Corresponding author: WANG Yan,
Received Date: 26 April 2007
Available Online: 26 June 2007

The optimum conditions of immobilized Lactate Oxidase with sodium alginate and ross-linked with glutaraldehyde was studied.The Lactate Oxidase was compared with its immobilized enzyme in some properties.Enzyme of 1 mL and 1 mL of 3% sodium alginate were mixed and the mixture was added dropwise into 20 mL of a 0.2 mol/L CaCl₂ solution to solidify for 2 h at 25℃.Then the precipitate was cross-linked in the solution of 20 mL of 0.2% glutaraldehyde for 2 h at 37℃.After filtration and drying,the immobilized enzyme beads were obtained with a activity recovery of 39.8% for the total enzyme added.The thermal stability of the immobilized enzyme was tested.The free enzyme lost all its activity when heated at 65℃ for 1 h but the immobilized enzyme still kept 86% of the original activity.The optimal reaction temperature of the immobilized enzyme was 55℃ compared to 37℃ of free Lactate Oxidase,the optimal reaction pH of the immobilized enzyme was 7.4 as the same as that of the free enzyme.Stored at 4℃ for 50 days without any protection by reagent,the free enzyme only kept 40% of the original activity but the immobilized enzyme still retained 80% of the original activity.The calcified preparation of pyruvate from DL-Lactate by immobilized Lactate Oxidase,the yield could reach 75% after 3 h,and the immobilized enzyme maintained 85% of the activity after having been reused 5 times.
- sodium alginate,
- immobilization,
- Lactate Oxidase,
- biocatalysis
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