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Citation: JIANG Hong, HU Xiao-Li. Fading Reaction of Acidic Dyes with Spectinomycin and Its Application to Analysis of Spectinomycin[J]. Chinese Journal of Applied Chemistry, ;2008, 25(4): 419-423. shu

Fading Reaction of Acidic Dyes with Spectinomycin and Its Application to Analysis of Spectinomycin

JIANG Hong ^a,, ,
HU Xiao-Li ^b

1.
a Department of Chemistry and Environmental Science, Changjiang Normal College, Chongqing 408003;
2.
b College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing

Corresponding author: JIANG Hong,
Received Date: 16 April 2007
Available Online: 26 August 2007
Fund Project: 教育部春晖计划资助项目(Z2005-1-55006) (Z2005-1-55006)重庆市教委科技基金资助项目(KJ051304) (KJ051304)

Under acidic condition,spectinomycin (SPM) was reacted with acid chrome blue K(ACBK),evans blue (EVB) or pontamine sky blue (POB) to form red or blue ion association complexes showing obvious fading peaks.The maximum fading wavelengths were at 522,608 and 626 nm,respectively,and the linear ranges were 0.2~5.0 mg/L (ACBK) and 0.1~5.4 mg/L (EVB or POB system),and their apparent molar absorptivities (ε) were 1.45×10⁴ L/(mol·cm),1.70×10⁴ L/(mol·cm) and 6.00×10³ L/(mol·cm),respectively.Under certain concentrations,the correlation of the spectinomycin concentration with the fading degree was found to obeyed Lombard Beer's law,and a new spectrophotometric method for the determination of spectinomycin (SPM) was thus developed.The optimum reaction conditions,the precision and reliability of methed and their applications were studied.The method was used for the determination of spectinomycin in injection samples and the recoveries of them were 100%,98.8% and 99.3%,respectively.
- spectinomycin,
- acid chrome blue K,
- evans blue,
- pontamine sky blue,
- fading reaction,
- spectrophotometry
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